Sex Difference and Testosterone Modulation: MATERIALS AND METHODS(4)

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Experiment 3: Effects of chemosensory cues and testosterone treatment on scent-gathering behavior. After they had received olfactory experience, subgroups of gonadec-tomized male (total n = 10) and female (total n = 10) ferrets continued to receive daily s.c. injections of TP or oil vehicle. Beginning 4 wk later, ferrets were tested individually on three separate days in a colony cage for scent-gathering and locomotor activity. During each test, either clean wood chip bedding or wood chip bedding that had been soiled for two days by either an estrous female or a male in breeding condition was placed on top of the slatted cage floor. Each subject was tested for 30 min on each type of bedding but received only a single type of olfactory stimulus on a given test day. antibiotic levaquin

A video camera was positioned directly above the cage to record each test session. These video tapes were later used to score the duration of scent-gathering behavior (sniffing) and the locomotor activity (grid crossings) displayed by each subject. Sniffing behavior is easily recognized as stereotyped head bobbing and movement of the nares. In order to facilitate the measurement of ferrets’ locomotor activity, white tape was placed on top of each test cage so as to divide it into 4 quadrants. An observer scored a grid crossing whenever a subject’s snout crossed one of the quadrant boundaries.