Sex Difference and Testosterone Modulation: MATERIALS AND METHODS(3)

8 Mar
2013

MATERIALS AND METHODS(3)

Such soiled bedding had induced a neuronal Fos-IR response in the MOB of gonadally intact male and female ferrets in breeding condition. Additional groups of subjects were placed in a plastic cage (24’’ X 8’’ X 8’’) for 30 min, whereupon peppermint odor was pumped into the cage by passing air (60 mm Hg) over imitation peppermint extract (Burns Philp Foods, Inc., San Francisco, CA) suspended in sesame oil. Finally, other groups of ferrets were placed in a clean colony cage with clean wood chip bedding beneath the slatted floor. Each of these stimulus conditions prevailed for 1.5 h, whereupon subjects were killed with an overdose (100 mg/kg) of sodium pentobarbital (JA Webster, Leominster, MA) and were perfused via the heart with 4% paraformaldehyde. birth control yasmin

Experiment 2: Neuronal Fos-IR responses in the chemo-sensory pathway after exposure to soiled bedding from a male in breeding condition. After they had received olfactory experience and had been used in a behavioral study (experiment 3), gonadectomized males (total n = 10) and females (total n = 7) received daily s.c. injections of TP (5 mg/kg) for 5 wk. Subgroups of males and females were then either placed in a colony cage that contained wood chip bedding previously soiled for two days by a male ferret in breeding condition or in a clean cage that contained clean wood chip bedding on top of the slatted floor. After 1.5 h, animals were killed with an overdose (100 mg/kg) of sodium pentobarbital and were perfused via the heart with 4% paraformaldehyde.

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