Similar relative trends were observed when tissue ho-mogenates were incubated with [3H]androstenedione. Interstitial glands from reproductively inactive females metabolized the [3H]androstenedione into predominantly testosterone (Table 3). The ovarian portion from these animals produced significantly less testosterone than the interstitial gland (p < 0.05), and it is possible that contaminating interstitial gland contributed to the testosterone produced. In addition, the ovarian portion synthesized estradiol and an unidentified ovary-specific metabolite (Table 3). Intact ovo-testes from the one reproductively inactive female produced testosterone, estradiol, and an ovary-specific metabolite at levels comparable to those observed for the respective portions when incubated separately (Table 3). Buy Advair Diskus Online
Intact ovotestes from pregnant females in spring produced significantly less testosterone than the interstitial gland from reproductively inactive females (p < 0.001, Table 3). Similar results were observed for the one nonpregnant female collected in spring (Table 3). Testes from reproductively active and inactive males synthesized only testosterone (Table 3).
|Testosterone (%)||Estradiol (%)||Ovarian metabolite (%)|
|Spring Ovotestis (pregnant)||10||33.8 ± 8.9||11.8 ± 7.7||34.6 ± 8.0|
|Spring Ovotestis (nonpregnant)||1||48.6||15.0||15.5|
|Autumn Ovary||3||73.0 ± 4.6||4.4 ± 1.6||4.3 ± 1.5|
|Autumn Interstitial Gland||3||83.3 ± 0.4*||nondetectable||nondetectable|
|Autumn Intact Ovotestis||1||83.8||3.64||4.0|
a Data are presented as percentage means ± SD and indicate the percentage of metabolized [3H]androstenedione that was present as testosterone, estradiol, and an unidentified ovarian metabolite. b Values given separately for each individual.
* Significantly different from spring ovotestis (pregnant) (p < 0.001) and autumn ovary (p < 0.05).