The one nonpregnant female collected in spring produced levels of testosterone that, per milligram of protein, were comparable to those of reproductively inactive females— reflecting the high levels of circulating testosterone observed in this animal (Table 1). The size of the ovotestes from this one nonpregnant female in the spring was within the range for those collected from the pregnant animals, but the structure was distinctive. In the nonpregnant individual the yellowish interstitial gland was distinct from the white ovary (follicular), whereas no such distinction could be made for the ovotestes of the 14 reproductively active (pregnant or postpartum) females. antibiotics levaquin
The testes of one of the two spring males examined contained more testosterone per milligram of gonadal protein than did those of males collected in autumn (Table 1). However, the testosterone concentration in the testes of the other spring male was considerably lower (Table 1).
When testosterone content per milligram of gonadal protein was corrected to testosterone content per total gonadal weight in milligrams, the increase in testosterone content for autumn ovotestis compared to spring ovotestis was highly significant (p < 0.001, Table 1). When individual testosterone contents per total gonadal weight were plotted against plasma testosterone, the animals fell into three distinct groups: spring pregnant females with the lowest values, autumn females and the one spring nonpregnant female (i.e., nonreproductive females) with significantly higher values (p < 0.001), and males with the highest values (Fig. 2).