Posts Tagged ‘Spermatid

Mutation of the Bruno-responsive elements in the oskar 3′ UTR results in the unregulated translation of the oskar mRNA. This unregulated translation requires the presence of functional Staufen protein. Stamen is an RNA-binding protein that may be associated with the cy-toskeleton and is involved in mRNA localization within the oocyte. If Staufen is missing, the […]

Although a number of different proteins have been implicated in linking transported and translationally controlled mRNAs to microtubules, only a few have been shown to bind directly to microtubules. Previous studies have shown that an RNA-binding activity specific for sequences present in the 3′ UTRs of a number of different mRNAs, including Prm2 and Tau, […]

We have shown by both light and confocal microscopy studies that the murine SPNR protein, which is associated with manchette microtubules in wild-type testes, is also present on the abnormal manchettes and microtubule aggregates found in the testes of four mouse mutants: azh, hop, tw8, and tw2. We never found localization of SPNR to be […]

A Coomassie-stained SDS-PAGE gel containing aliquots of each supernatant and pellet is shown in Figure 7A. The vast majority of the added tubulin is pelleted under these conditions, and most of the starting material remains in the first supernatant. Figure 7B shows a corresponding Western blot probed with the a-His-tag antibody that recognizes the E. […]

To test whether a recombinant His-tagged version of SPNR produced in E. coli could be purified with testis microtubules, as is the endogenous SPNR protein, testis microtubules were pelleted and resuspended in SPNR-con-taining E. coli extracts prepared as described in Materials and Methods. These microtubule-enriched extracts were subjected to two further rounds of centrifugation and […]

Microtubule Binding InVitro The SPNR protein is coprecipitated with murine testis microtubules and its associated proteins. Extracts prepared from as few as four mouse testes (approximately 400 ^g tissue) were incubated at 37°C in the presence of GTP and the microtubule-stabilizing drug taxol and then centrifuged at 100 000 X g to pellet microtubules. The […]

Male mice homozygous for the tw2 haplotype are sterile. Although many of the seminiferous tubules degenerate and are devoid of germ cells, as seen in the tw8 haplotype, other seminiferous tubules have an abnormal phenotype that is distinct from that in tw8/tw8 testes (Fig. 1). Previous studies with tw2/tw2 testes have shown that the nuclear […]