Posts Tagged ‘Ribonucleic Acid

Microtubule Purification from Mouse Testes Microtubules were purified from mouse testes by a taxol-dependent method modified from Vallee. Testes were dissected from sexually mature Swiss Webster male mice, and the tunicas were removed. Approximately 400 ^g of tissue was resuspended on ice in 1 ml microtubule (MT) buffer (0.1 M Pipes, pH 6.6, 1 mM […]

Mice Azh mice were obtained from The Jackson Laboratories (Bar Harbor, ME). Hop sterile (hop) mice were provided by Dr. Mary Ann Handel, University of Tennessee, Knoxville, TN. Mice carrying the tw8 and the tw2 haplotypes were from the T/t complex colony at the University of Texas at Austin. All animal experiments were conducted in […]

We have previously described an RNA-binding protein in mammalian spermatids whose association with the man-chette suggests that it may be involved in the transport or translation of selected mRNAs. The gene encoding the spermatid perinuclear RNA-binding protein (SPNR) was cloned in a screen for RNA-binding proteins that could bind to the 3′ untranslated region (UTR) […]

Two possible and not mutually exclusive functions for the manchette are to provide the mechanical force necessary to mediate nuclear shaping and to act as a “track” for the movement of cellular components into the extending cytoplasm. Nuclear shaping is under genetic control, and drugs that affect the manchette, including taxol, cytoxan, and 5-fluorouracil, also […]

Formation of a spermatozoon from a diploid spermato-gonial progenitor cell involves reduction of the chromosomal content during meiosis and several major structural changes to the cell during spermatid differentiation. These changes, which include compaction of the chromatin, changes in nuclear morphology, development of the acro-some, and formation of a flagellum, require the expression of testis-specific […]

Numerous studies addressing the in vitro RNA-binding properties of different members of this family of proteins suggest that they are sequence-independent RNA-binding proteins that prefer double-stranded RNAs and highly structured single-stranded RNAs. Northwestern analysis with an SPNR-maltose-binding protein fusion showed that it too can bind to a number of different mRNAs in vitro. Despite the […]