Posts Tagged ‘Implantation

Isolation of the PEP8 (peptidase-deficient) mutant in yeast revealed 44% identity with the Hp58 gene in mouse. The defect in yeast was not lethal and overexpression of PEP8 did not affect cell viability. Analysis of the protein distribution showed immunolocalization to the yeast vacuole (equivalent of the mammalian lysosome). Domain swap experiments between the carboxyl […]

Placental defects that impair further development of the conceptus can be due to developmental errors in particular cell lineages or to faulty vascularization in the placenta. Because the H358 mutation was lethal in early postimplantation development, any requirement for its function in later pregnancy could not be assessed in the mutant mice. The present study […]

The data reported here indicate that the expression of a protein with sequence similarity to H358 is spatially and temporally regulated during the formation and maturation of the chorioallantoic placenta in the rat. During pregnancy, H358 is differentially expressed in the maternal endometrium, decidua, and fetal placental cells. The H358 in the fetal placenta was […]

Expression of H/358 in Cultured Placental Cell Lines The HRP/LRP trophoblast stem cells provide an important source of labyrinthine trophoblast cell precursors from which to obtain additional information about placental cell lineages. We therefore determined whether cell lines isolated from the labyrinthine placenta (HRP/LRP) and postulated to contain trophoblast stem cells expressed Hp58. The amount […]

H/358 mRNA Is Preferentially Expressed in the Labyrinth Region of the Mature Chorioallantoic Placenta As the chorioallantoic placenta matured between Days 10 through 14, the labyrinth zone became highly vascularized and contained a large number of Hp58-positive fetal erythroid cells. In addition, at Day 14 of pregnancy Hp58 was detected in the syncytiotrophoblast of the […]

At Day 12 of pregnancy two separate populations of trophoblast giant cells were identified; however, Hp58 expression was not detected in trophoblast cells at either of these stages. As maturation of the chorioallantoic placenta progressed to Day 14 of pregnancy, Hp58 was detected in the trophoblast giant cells, and it was particularly evident in the […]

Localization of H/358 Protein in the Fetal Placenta Partitioning of the fetal rat placenta into two morphologically distinct regions termed the labyrinth and junctional zones becomes evident early in chorioallantoic placental development. Because mice lacking functional Hp58 failed to form a chorioallantoic placenta, it was essential to investigate the distribution of Hp58 in the different […]