Posts Tagged ‘Estrogen

Immunohistochemistry Nuclear ER protein was localized in neonatal uterine tissue sections using the mAb H222 as described previously. Procedures for localization of ER in paraffin-embedded tissue sections were modified from those described by Cartun and Pederson and Papadimitriou et al.. Sections were deparaffinized in xylene, rehydrated through a series of ethanol washes, and rinsed in […]

Western Blotting ER protein was detected on Western blots and by immunohistochemistry using the H222 rat anti-human ER monoclonal antibody (H222 mAb; gift of Dr. G.L. Greene, University of Chicago). The precise epitope recognized by the H222 mAb is not known. Though preliminary, current evidence indicates that this antibody is specific for ERa (Dr. G.L. […]

Animals and Tissue Collection At birth (Day 0), crossbred gilts were assigned to remain intact (n = 30) or to be ovariectomized (OVX, n = 25) on Day 0. Animals in each treatment group were then assigned to be hysterectomized (n = 5 gilts per day) under general anesthesia on either Day 0 (intact only), […]

Effects were related to age at first exposure. Uterotropic effects of EV were confined largely to the endometrium on PND 7 and 14 and, overall, were greater in older animals . Age-specific responses of neonatal porcine uterine tissues were interpreted to suggest that acquisition of estrogen sensitivity occurs progressively in the developing uterine wall and […]

In the pig, endometrial glands are absent at birth but develop rapidly thereafter, forming coiled, branched epithelial tubes that extend radially from the luminal surface, through endometrial stroma, to the adluminal border of the myometrium by approximately PND 30. Intensely glandular folds of endometrium are apparent by PND 60. Events that accompany the appearance and […]

Organizational mechanisms regulating growth, morphogenesis, and cytodifferentiation of uterine tissues are poorly defined. However, it is clear that adult uterine structure and function can be compromised if developmental events critical to the success of uterine wall organization are disrupted by exposure of tissues to estrogen or related compounds during specific periods of fetal or neonatal […]