Posts Tagged ‘Embryos

These results also suggest that the presence of binucleate cells observed in the present study is due to abnormal polymerization of actin filaments, which results in nuclear division without cytoplasmic division. In addition, asynchronized nuclear and cytoplasmic division (as shown in Figs. 4 and 5) indicates that culture conditions may delay the progression of polymerization […]

In the present study, through a comparison of in vivo-derived and in vitro-pro-duced embryos, we also found that microfilament distribution was significantly different between these two sources of embryos, especially in intracellular actin filaments. Recently it was reported that intracellular actin filament distribution was directly related to the 2-cell block in hamster and rat embryos. […]

Interestingly, recently it has been found that addition of glutathione during the sperm-oocyte reaction and embryo culture significantly improved blastocyst formation without increasing intracellular glutathione. It is possible that glutathione can also reduce ROS in the culture medium and thus protect the embryos against oxidative stress. It was also found that glutathione was present in […]

Recently it was also reported that epidermal growth factor or coculture of pig oocytes with follicle shell pieces improved cytoplasmic maturation of pig oocytes by improving male pro-nuclear formation and subsequent embryo development. In the present study, we used an in vitro system that results in more than 30% of oocytes matured and inseminated to […]

In the present study, about 80% of inseminated oocytes were penetrated by sperm, and more than 90% of penetrated oocytes formed both male and female pronuclei. However, 35% of fertilized oocytes were polyspermic. From cleavage rates at 36 h and 48 h after IVF, we estimated that most fertilized oocytes that had formed both male […]

Embryos at the 2- to 4-cell stage did not develop in medium with cytochalasin D, indicating that actin filaments play an important role in embryo development. The present study is the first detailed examination of morphology of pig embryos produced by IVM/IVF/IVD. Although 58.3% of cleaved embryos developed to the blastocyst stage under present conditions, […]

When in vitro-produced one-cell embryos (6 h after IVF) were transferred to oviducts of the recipient animals and then flushed 5 days after transfer, the recovered blastocysts had more than 100 nuclei. However, when the embryos were cultured in vitro, development was delayed (about 1 day), and nuclear number in the blastocysts was decreased, present […]