Posts Tagged ‘Antimicrobial peptide

AD is multifactorial, polygenetic skin disorder and is commonly viewed as immunologic in patho­genesis. Examination of skin biopsy samples from patients with AD has shown that S. aureus grows in colonies in the upper layers of the epidermis between keratinocyres. This suggests that an exponential increase in S. aureus could results from failure of the […]

1) TEWL measurement between involved and uninvolved skin For comparisons of TEWL levels between involved and uninvolved sites in AD, measurements were performed 5 times for each site, repeatedly. The mean TEWL level of healthy donor was 7 g/hm2. The mean TEWL levels increased in both un­involved and involved sites than that of healthy donors, […]

Electrophoresis The products were run on 1.5% agarose gel contain 1 yg ethidium bromide per millimeter. 20 yl of reaction mixture was mixed with loading buffer, separated by electrophoresis for 15 minutes at 100 voltages and visualized by UV transillumination. Quantitative analysis PCR products of hBD-2, LL-37 were normalized with GAPDH on DIG chemiluminescent film […]

Subjects Punch skin biopsies were taken from involved and adjacent uninvolved skin of 7 patients with AD after TEWL measuring. AD was diagnosed according to the standard criteria(Hanifin and Rjaka, 1982). Normal skin was obtained from healthy donor. Informed consent was obtained from all subjects after they were fully informed about the details and the […]

INTRODUCTION Atopic dermatitis (AD) is a highly pruritic recurring inflammatory skin disease. It can be ex­acerbated by bacterial, viral, and fungal infections. In fact, many AD patients suffered from recurrent infections of skin lesions. Especially, Staphylococcus aureus (S. aureus) is found in more than 90% of AD skin lesions, but in only 5% of normal […]