The relative levels of mRNA and proteins corresponding to the expression of key enzymes involved in the biosynthesis of prostanoids were measured in bovine myometrium. One important feature of our approach was the use of primary cultures of separated myometrial layers. We have shown that the two smooth muscle layers have distinct patterns of PG production, and exploratory studies in our laboratory suggested that a subculture of myocytes could alter PGHS gene regulation (data not shown). proventil inhaler
Our study shows that the modulation of PG synthesis in the myometrium parallels some changes in cPLA2. The reduced mobility of the band suggests that the phosphorylated form, corresponding to the fully active enzyme, is stimulated by PMA. Consequently, after PMA treatment, increased PG production would be favored by increased availability both of the AA substrate through cPLA2 and of its PGHS-2-converting enzyme. No rapid induction of PGHS-2 mRNA comparable to that with 6-h PMA treatment was observed after oxytocin treatment (data not shown). Our results describe both differences and similarities in the regulation of PG synthesis in the two myome-trial layers.