Spermatid Perinuclear Ribonucleic Acid-Binding Protein Binds Microtubules In Vitro and Associates with Abnormal Manchettes In Vivo in Mice(2)

30 Jan

Two possible and not mutually exclusive functions for the manchette are to provide the mechanical force necessary to mediate nuclear shaping and to act as a “track” for the movement of cellular components into the extending cytoplasm. Nuclear shaping is under genetic control, and drugs that affect the manchette, including taxol, cytoxan, and 5-fluorouracil, also affect sperm head shape. Several mutations in the mouse that result in reduced fertility or sterility are characterized by highly abnormal manchettes and altered head shape, suggesting that the manchette functions in nuclear shaping. It has also been suggested that the manchette could serve to transport cytoplasmic constituents caudally, an event that coincides with manchette appearance. flovent inhaler

Manchette microtubules have been shown to be associated with vesicles and the endoplasmic reticulum, and immunocytochemistry studies in the rat have shown that the microtubule motor proteins kinesin and dynein are both found on the man-chette. Other proteins have also been localized to the manchette, including the microtubule-associated protein (MAP) Tau, a protein kinase, and the intermediate filament protein Sak57.