Age-related changes in ER immunostaining intensity observed for GE, LE, and S are summarized in Figure 5. Nuclear staining was absent in endometrium from PND 0. However, by PND 15, with the appearance of endometrial glands, strong positive nuclear staining was observed in GE. In striking contrast, nuclear ER staining was effectively absent in LE on PND 15, although a few individual LE nuclei did stain weakly for ER protein. Strong nuclear ER staining was observed for GE in all tissues obtained on and after PND 15. Staining of LE nuclei was weak but more regular in tissues from PND 30 and 60 and was moderate but regular in tissues from PND 90 and 120. Stromal cell nuclear staining for ER was weak to moderate on PND 15, moderate on PND 30, and strong thereafter. Buy Asthma Inhalers Online
Positive nuclear immunostaining, indicating presence of ER protein, was observed only when the H222 primary antibody was applied to Pronase-treated tissue sections. No nuclear staining was observed when irrelevant rat IgG was substituted for the H222 primary antibody or when primary antibody was omitted, nor was nuclear staining observed in sections of porcine small intestine when H222 was applied as described above (data not shown).
FIG. 5. Summary of effects of postnatal age, from birth (Day 0) to PND 120, on nuclear staining intensity for ER protein as detected immuno-histochemically using the H222 antibody (see Fig. 4). Staining intensity was scored visually in multiple tissue sections from each gilt (n = 5 per day) in endometrial CE, LE, and S. Relative staining intensity levels were designated 0 (absent, i.e., no nuclear staining), 1 (weak), 2 (moderate), or 3 (strong). ER-specific nuclear staining was absent in tissues from PND 0.