Ovary-Independent Estrogen Receptor Expression: RESULTS(1)

18 Jan
2013

Uterine Growth

Effects of age and OVX at birth on uterine wet weight and endometrial thickness are summarized in Figure 1. Both uterine weight and endometrial thickness were affected by age and OVX (p < 0.01), and an interaction (p < 0.01) between age and OVX was detected for both of these dependent variables. Overall, OVX at birth did not affect patterns of change in uterine weight or endometrial thickness between birth and PND 60. Both parameters increased (p < 0.01) in a similar way in intact and OVX gilts before PND 60. After PND 60, these values were greater and increased (p < 0.01) more rapidly in intact gilts (Fig. 1). However, both uterine wet weight and endometrial thickness continued to increase in OVX gilts to PND 120.

Histology

Photomicrographs depicting age-related changes in endometrial histology between birth and PND 120 in intact and OVX gilts are shown in Figure 2. On Day 0, the porcine endometrium had no glands but consisted of a simple columnar epithelium overlying developing stroma. By PND 15, GE was present and tubular glands had begun to coil and penetrate the endometrial stroma. Between PND 15 and PND 120, endometrial folds developed and uterine glands continued to proliferate such that the deep stratum spongiosum became intensely glandular. OVX at birth did not inhibit uterine gland genesis. buy diabetes drugs
fIG1Ovary-independent estrogen
FIG 1. Effects of neonatal age and OVX at birth on porcine uterine wet weight (A) and endometrial thickness (B). Both uterine wet weight and endometrial thickness were affected by postnatal age (p < 0.01) and OVX (p < 0.01), and an age x OVX interaction was detected (p < 0.05). For each response, least-squares means (± SE) for intact (solid circles) and OVX (open circles) gilts are presented, together with best-fit least-squares regression curves. Patterns of change in uterine wet weight (A) followed a cubic trend for intact (Y = -0.003 + 0.203X – 0.006X2 + 0.00006X3; R2 = 0.97, p < 0.01; solid line) and a linear trend for OVX gilts (Y = —0.136 + 0.097X; R2 = 0.74, p < 0.01; broken line). Patterns of change in endometrial thickness (B) followed different quadratic trends for both intact (Y =259.5 + 4.73X + 0.034X2; R2 = 0.87, p < 0.01; solid line) and OVX gilts (Y = 288.4 + 10.3X – 0.05X2; R2 = 0.62, p < 0.01; broken line).

fIG2Ovary-independent estrogen
FIG. 2. Representative photomicrographs depicting histogenesis of the porcine endometrium from birth (DO; top) through PND 120 (D120; bottom). Uteri were obtained at hysterectomy on Days 0, 15, 30, 60, 90, and 120 from both intact gilts and gilts OVX at birth. Tissue sections were stained with Harris hematoxylin and eosin. Note the aglandular endometrium on DO. GE is present by PND 15, and glands proliferate throughout the S as animals age. M = myometrium. Bar = 100 jj,m.

top