Rat Amnion Type IV Collagen Composition and Metabolism: RESULTS(3)

7 May

In agreement with our previous zymo-graphic studies, MMP-2 (66-kDa lysis band) and MmP-9 (92- and 180-kDa lysis bands) activities were increased on Day 21 compared to Day 19 (Fig. 3D). A lysis band at 68 kDa, possibly reflecting the activated form of MMP-9, was also detected on Day 21. A faint lysis band at 31 kDa was found consistently in amnion samples collected on Days 18 and 19 but was absent on Day 21. antibiotics levaquin

Infection of Rat Amnion Organ Cultures with a Recombinant Adenovirus Expressing proMMP-9 Promoted Collagenolysis and Amnion Cell Apoptosis Altering expression of MMP-9 results in profound changes in extracellular matrix catabolism and cellular characteristics, including cell death. Transfection of rat embryo cells with an MMP-9 expression vector produced cells with a metastatic phenotype, a reflection of increased capacity to degrade extracellular matrix components. Conversely, inhibition of MMP-9 expression in transformed cells that constitutively produce MMP-9 by using a ribozyme suppressed metastasis without affecting tu-morigenesis.

To test the hypothesis that induction of a type IV collagen-degrading activity causes collagen degradation, cell detachment, and subsequent cell death, we infected organ cultures of rat amnion collected on Day 18 with recombinant adenovirus expressing proMMP-9 (AdMMP-9) or with a control virus (AdLacZ) expressing LacZ (5.2 X 108 pfu/ well).