AMEBIASIS AND COMPARISON OF MICROSCOPY: RESULTS

23 Dec
2009

The analysis of the records of amoebal infection in various hospitals in Kilimanjaro indicated frequent occurrence of amebiasis. The results of this study indicated that the population over the age of five years had a higher rate of amoebal infection compared to the population under the age of five years. However, both age groups had similar patterns of amoebal infection throughout the study period (Figure 1). This study indicated that females over the age of five had a higher rate of infection during February-March of 2000. However, during February-March of 2001, males over the age of five had a higher rate of infection, which remained high until the middle of the year; otherwise, there was no significant difference in the amoebal infection between the male and female populations (Figure 2).

Figure 1. Percent rate of amoebal infection in the different

Figure 1. Percent rate of amoebal infection in the different age groups among the people of Kilimanjaro, Tanzania. The rate of amoebal infection was higher in persons older than five years. However, the infection rate pattern was the same in both groups.
Table 1. Detection of amoebal infection (histolytica/dispar) among 842 subjects using Entamoeba Test (TechLab). The sensitivity (39%) and specificity (96%) were determined using dichotomous approach. The amoebal infection was 8.2%, and infection missed by microscope was 61%.

Test Results     Infected   Not Infected

Total

Positive            27            32

59

Negative          42          741

783

Total                69           773

842

Sensitivity = 39%; Specificity = 96%
Positive predictive value = 46%
Negative predictive value = 95%
Infection missed by microscope:
1- sensitivity = 61%
Fraction unnecessarily treated:
1 – specificity = 4%
Fraction with amoebal infection
(dispar/histolytica) = 8.2%

The frequency of E. histolytica infection in both passive and active cases was 1%, while the prevalence of E. dispar was 7.3% among passive cases and 15% among active cases. tadalis sx 20

Figure 2. Showing the differences

Figure 2. Showing the differences in the percent rate of amoebal infection between male and female populations of Kilimanjaro, Tanzania. Both sexes had the same pattern of amoebal infections. The amebiasis was more prevalent during 2000 and 2001, compared to 1999.

Table 2. Detection of E. histolytica infection among 842 subjects using E. histolytica-\\ Test (TechLab). The sensitivity (43%) and specificity (93%) were determined using dichotomous approach. The E. histolytica infection was 0.8%, and infection missed by microscope was 57%.

Test Results     Infected   Not InfectedTotal
Positive              3              56

59

Negative            4            779

783

Total                  7            835

842

Sensitivity = 43%; Specificity = 93%
Positive predictive value = 5%
Negative predictive value = 99.5%
Infection missed by microscope:
1 – sensitivity = 57%
Fraction unnecessarily treated:
1 – specificity = 7%
Fraction with E. histolytica infection = 0.8%

The detection of amoebal infection with the microscopy was compared to the ELISA test. The sensitivity and specificity of microscopy technique were 39% and 96%, respectively, while the PPV and NPV were 46% and 95%, respectively. The total amoebal infection was 8.2%, and the detection of amoebal infection missed by microscopy was 61% (Table 1). In detecting only E. histolytica infection, microscopy sensitivity and specificity were 43% and 93%, respectively. The PPV and NPV were 5% and 99.2%, respectively. The total E. histolytica infection was only 0.8%, and the detection of E. histolytica infection missed by microscopy was 57% (Table 2). In detecting only E. dispar, the sensitivity and specificity were 39% and 95%, respectively. The PPV and NPV for E. dispar were 41.1% and 94.7%, respectively. The total E. dispar infection was 7.4%, and the detec tion of E. dispar infection missed by microscopy was 61%. (Table 3). This study indicated that E. dispar infection was 14.5 times more prevalent than E. histolytica infection, and the proportion of E. histolytica infection in the population is 7%. online canadian pharmacy

Table 3. Detection of E. dispar among 842 subjects using Entamoeba Test minus E. histolytica-\\ Test (TechLab). The sensitivity (39%) and specificity (96%) were determined using dichotomous approach. The E. dispar infection was 7.4%, and infection missed by microscope was 61%.

Test Results     Infected   Not InfectedTotal
Positive            24            35

59

Negative          38          745

783

Total                62           780

842

Sensitivity = 39%; Specificity = 96%
Positive predictive value = 41%
Negative predictive value = 95%
Infection missed by microscope:
1- sensitivity = 61%
Fraction unnecessarily treated:
1 – specificity = 4%
Fraction with E. dispar infection = 7.4%
top