Adrenalectomy in rats depresses hypoxic pulmonary vasoconstriction in vitro but does not attenuate the pulmonary hypertension of chronic hypoxia (part 1)

10 Aug
2012

hypertension of chronic hypoxia (part 1)

Stimulation of glucocorticoid receptors has been found to increase vasoreactivity of isolated rat lungs perfused with either physiological salt albumin solution or blood obtained from adrenalectomized rats. Small doses of dexa-methasone potentiated the pressor responses of isolated lungs to airway hypoxia and to bolus injections of angiotensin II . In the present study we asked whether adrenalectomy alters the reactivity of pulmonary vasculature. Two experiments were performed in rats. First, we studied in isolated rat lungs the effect of adrenalectomy of lung donors on acute hypoxic pulmonary vasoconstriction and on vaso-constrictions induced by angiotensin II injections. In the second experiment we evaluated the in vivo effects of acute and chronic hypoxia on pulmonary hemodynamics in intact ad-renalectomized rats.ANIMALS AND METHODS Experimental animals: Fifty-one Wistar male rats were divided into three pairs of litter mate groups. In each pair, one group was adrenalectomized and the other underwent sham surgery. The first pair was used in an acute experiment (lung and blood donors for isolated lung preparation); the next pair of groups was exposed to chronic hypoxia and the last pair of groups was kept in normoxia. Bilateral adrenalectomy was performed through retroperitoneal incisions under ether anesthesia. Our highly experienced health experts recommend visiting the my canadian pharmacy.com whenever you need a cheap and efficient medication, because this pharmacy offers the best choice of efficient meds delivered quickly to your doorstep.

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