Morphologic Evaluation and Actin Filament: RESULTS(1)

22 May
2013

Morphology of Pig Embryos Produced In Vitro (Experiment 1)

As shown in Table 1, no statistical differences were observed in the sperm penetration rates among the examination time points from 12-48 h after IVF. At 12 h after IVF, 93% of fertilized oocytes had formed both male and female pronuclei and released the second polar body. Of fertilized oocytes, 35% were polyspermic. No cleaved embryos were observed at 12 h after IVF. Cleaved embryos were first observed 24 h (25%) after IVF, and the cleavage rate had significantly increased at 30 h (71%) after IVF and reached the highest point at 36 h (83%) after IVF. Cell stages (number of blastomeres) progressed as the culture time increased (Table 1). The detailed nucleus-blastomere relationship at 2- to 4-cell stages is shown in Figure 1A. antibiotics levaquin

From a total of 269 embryos examined, 109, 19, 74, and 67 were at the 2cell, 3-cell, 4-cell, and 5- to 8-cell stages, respectively. Of the embryos examined, as shown in Figure 1B, 73% of 2-cell, 26% of 3-cell, 49% of 4-cell, and 26% of 5- to 8-cell embryos were normal in morphology; the proportion of embryos with abnormal morphology increased as number of blastomeres increased, from 27% at the 2-cell stage to 74% at the 5- to 8-cell stage.

TABLE 1.

Time (h)afterIVFNo. of oocytes examinedNo. of oocytes penetrated (%)aNo. of oocytes cleaved (%)bNo. of embryos at the following stages:
V ^ 2( 3-cell(%)c 4-cell(%)c 5-8-cell(%)c
12125100 (80 ± 9)0 d
2412090 (75 ± 4)24 (25 ± 10)e12 (72 ± 16)d2 (5 ± 5)d7 (17 ± 10)d3 (7 ± 7)d
30140116 (82 ± 9)87 (71 ± 8)f49 (57 ± 8)d3 (5 ± 2)d30 (31 ± 7)e5 (8 ± 4)d
3612797 (74 ± 9)84 (83 ± 6)g29 (35 ± 10)e3 (3 ± 2)d22 (33 ± 9)e30 (30 ± 8)e
4812289 (73 ± 5)74 (83 ± 3)g19 (26 ± 4)e11 (15 ± 8)e15 (21 ± 6)de29 (39 ± 9)e

a Percentage of the number of oocytes examined. b Percentage of the total number of oocytes penetrated. c Percentage of the number of oocytes cleaved.
defg Values with different superscripts within the same column are significantly different, p < 0.05.

Fig1Morphologic Evaluation and
FIG. 1. Morphological characteristics of pig embryos produced in vitro. Embryos were examined 24, 30, 36, and 48 h after IVF. A) Morphological characteristics of 2- to 4-cell-stage embryos. Four different morphological characteristics were observed; for details, also see text. B) Summarized results. C) The relationship between the culture duration and proportions of embryos with normal morphology. abc Significantly different among the stages of embryos, p< 0.05.

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