Morphologic Evaluation and Actin Filament: MATERIALS AND METHODS(5)

21 May


The image was obtained by repeated laser scanning (5 times during 5 sec) to improve the signal-to-noise ratio.

Effect of Cytochalasin D on Embryo Development and Microfilament Distribution (Experiment 3)

In experiment 3, in order to further examine the effect of microfilaments on embryo development, 2- to 4-cell embryos selected at 36 h after IVF were cultured in medium with or without 5 ^M cytochalasin D, an inhibitor of microfilament polymerization. At 4.5 days (6 days after IVF) after culture, blastocyst formation and actin filament distribution were examined by methods described above. antibiotic levaquin

Statistical Analysis

Four replicate trials were conducted for experiments 1 and 3, and all percentage data were subjected to arc sine transformation before statistical analysis. Comparisons were conducted by ANOVA. For examination of actin filament distribution, embryos at 36 h and 6 days (blastocyst) after IVF from four different experimental trials were used. Cell numbers in embryos from in vivo and in vitro procedures were directly compared by ANOVA. A value of p < 0.05 was considered to be statistically significant.