Accumulation of Cyclooxygenase-2 Gene Transcripts: RESULTS(3)

7 Apr


The fetal membranes, chorioallantois, and amnion from cows at term expressed COX-2 mRNA, but the signals were weaker than in cotyledons (not shown).

Figure 2 shows a representative gel for the protected fragments of COX-2 mRNA and GAPDH in intercarun-cular endometrium, myometrium, and cervical mucosa. COX-2 mRNA expression in endometrium was variable and rather weak during pregnancy and parturition, but increased dramatically postpartum. The patterns of COX-2 mRNA expression in myometrium and endometrium were similar with no specific trend during pregnancy; but postpartum, no increase in the concentration of COX-2 transcripts was observed in the myometrium. antibiotics levaquin

One of the three cows in active labor showed a more robust signal for COX-2 mRNA in myometrium than during pregnancy. In cervical mucosa the concentration of COX-2 mRNA was very weak on Days 150 and 275, but at term the signal for COX-2 transcripts had increased before the onset of labor and was strong in parturient cows. The expression of COX-2 mRNA in cervical mucosa declined after delivery; at 6-12 h postpartum, levels of transcripts were similar to those at term before the onset of labor. Figure 3, A and B, shows the results expressed as the intensity of COX-2 mRNA signals in percentage of the corresponding GAPDH mRNA signal.
Fig2Accumulation of CyclooxygenaseFIG. 2. RPA for COX-2 mRNA in samples of endometrium, myometrium, and cervical mucosa obtained from the same cows as the placental tissues shown in Figure 1. Messenger RNA from endometrium is in lanes 1-12, mRNA from myometrium in lanes 13-23, and mRNA from cervical mucosa in lanes 24-29; stage of gestation as indicated. For comparison, endometrium from two cows at estrus was also assayed (d 0).

Fig3Accumulation of CyclooxygenaseFIG. 3. Quantification of protected fragments for COX-2 mRNA and GAPDH mRNA shown in Figures 1 and 2 performed using a Laser Densitometer (Advanced American Biotech Imaging) to measure areas of the protected fragments on the films. Values shown are mean ± SE for relative intensities of COX-2 mRNA as percentage of corresponding GAPDH mRNA; n = 2 except for cervix on Days 0, 275, 280-, 280+ (n = 1). RPA using RNA from the remaining cows, one or two each day, was performed separately with essentially the same results. A) Endometrium, myometrium, and cervical mucosa: concentrations of mRNA for COX-2 in endometrium from postpartum cows and cervical mucosa from cows in labor had increased significantly from initial values (on Day 150 and Day 2 75, respectively [ANOVA, p < 0.05]); no significant changes occurred in COX mRNA concentrations in samples from myometrium. Endometrium from two estrous cows were analyzed for comparison (Day 0). B) Caruncles and cotyledons: COX-2 mRNA concentrations on Days 280- and 280+ were significantly greater in both tissues than on Day 150 or postpartum; concentration of COX-2 mRNA in cotyledons on Day 2 75 was also greater than on Day 150 (ANOVA, p < 0.01).