Accumulation of Cyclooxygenase-2 Gene Transcripts: RESULTS(1)

5 Apr

RESULTS(1)COX-1 Expression in Tissues of Pregnant Cows

No signals for COX-1 mRNA were detected by RPA in 20 ^g of total RNA from tissues of pregnant cows after exposure on XOMAT-AR (Eastman Kodak, Rochester, NY) films for 1 wk. After exposure for 3-7 days on BioMax (Eastman Kodak, Rochester, NY) film, which is three times as sensitive as XOMAT-AR, signals for COX-1 mRNA were sporadically present in RNA from caruncles, endometrium, and myometrium; the relative intensity vs. buy cheap antibiotics

GAPDH mRNA at parturition was about 3.5% in caruncles, less than 0.1% (trace) in intercaruncular endometrium, and 7.3% in myometrium. Signals for COX-1 mRNA were not detected in RNA from cotyledon, cervical mucosa, and chorioallantois. COX-1 mRNA signals in 20 ^g total RNA from seminal vesicular and prostate glands from a fertile bull, used as positive controls, were also weak; the relative intensities vs. GAPDH mRNA were 8.3 + 1.0% and 2.6 ± 0.5%, respectively, in three separate assays. Using RT-PCR, COX-1 cDNA was detected in seminal vesicular and prostate glands, myometrium, and caruncle from parturient cows, as well as in the fetal uterus but not the fetal cervix. No further RPAs were performed for COX-1 in bovine tissues.