Archive for the ‘Steroid Hormones’ Category

Because the levels of PGI synthase are comparable in the two muscle layers and do not vary with steroid treatments, it is tempting to conclude that the regulation of PGI2 production in the myometrium is regulated through the expression of PGHS-2. It is also interesting if not intriguing to see that the selective PGHS-2 inhibitor […]

While increased expression of PGHS-2 in response to PMA is more evident in the longitudinal layer, Although we have not observed any modulation of PGHS-1 gene expression in the cyclic bovine myometrium, the role of this enzyme in the regulation of other reproductive processes such as parturition remains to be elucidated. There is good evidence […]

The relative levels of mRNA and proteins corresponding to the expression of key enzymes involved in the biosynthesis of prostanoids were measured in bovine myometrium. One important feature of our approach was the use of primary cultures of separated myometrial layers. We have shown that the two smooth muscle layers have distinct patterns of PG […]

It is interesting to note that the effect of steroid hormones on PGHS-2 protein or mRNA level correlates well with PG accumulation in both myometrial layers (Table 1). Indeed, compared to those in the control group (6-h treatment with PMA in the absence of steroid), PG levels were not affected by E2 treatment whereas P4 […]

Effect of Exogenous Sex Steroid Hormones on Distinct PG Synthesis Pathways in Bovine Myometrium The effects of steroid hormone E2 alone or in combination with P4 on PGHS-2 gene expression were assessed (Fig. 2). Myocytes from the circular and the longitudinal layers of bovine myometrium were treated with 1 nM E2 (72 h) and/or 10 […]

PGHS and cPLA2 Protein Expression in Response to PMA in Cultured Myocytes Figure 1A shows the effect of PMA on the pattern of PGHS-1, PGHS-2, and cPLA2 proteins in the two smooth muscle layers of the bovine myometrium. Under basal conditions, without PMA stimulation, two immunoreactive bands were detected after immunoblotting with antiserum directed against […]

Total RNA (10 ^g) was loaded on a 1% agarose-2.2 M formaldehyde gel, transferred to nylon membranes (Magna; Micron Separations Inc., Westboro, MA), and then cross-linked by UV irradiation. After 4 h of prehybridization in a 50% formamide solution at 42°C, membranes were sequentially hybridized overnight at 42°C in the same solution containing [a-32P]dCTP-labeled cDNA […]