Archive for the ‘Pleural Fluid’ Category

In earlier studies, concentrations of C3d, C4d, and Bb fragments in pleural fluid have been higher in patients with tuberculous pleurisy than in patients with malignant pleurisy. In our study, pleural fluid C3d was significantly and Bb was almost significantly higher in tuberculous pleurisy than in malignant pleural effusions, but no significant difference was seen […]

Patients with rheumatic effusion consistently showed a high level of pleural SC5b-9, in contrast to those with malignant effusions, and this is compatible with the results of a previous study that showed that SC5b-9 was >2.0 mg/L in two patients with rheumatic effusion and in none with malignant pleural effusion. They also showed that SC5b-9 […]

The higher concentrations of several activation products in the pleural fluid compared with those in plasma could suggest that immune complex activation is taking place in the pleural cavity and the activation products are not leaked from the peripheral blood. Previously it has also been suggested that circulating immune complexes themselves may lead to pleural […]

Hara et al confirmed earlier findings and showed that the soluble terminal-phase complement complex (SC5b-9) was the most effective way to differentiate between tuberculous and malignant pleural effusions. In the present study, we have used both these methods, and other additional markers, to find out whether the analysis of complement components and their activation products […]

In rheumatic pleural effusion, the concentrations of C3 and C4 were also significantly (p—0.020, p<0.001, respectively) lower than in tuberculous pleurisy, but there was no significant difference in the concentrations of C3d, C4d, or Bb. However, the overall activation of the complement system showed a significant difference (p=0.003); the mean level of SC5b-9 was 29.3 […]

Comparisons between groups with different etiology were performed with the Kruskal-Wallis and Mann-Whitney tests. Analyses of the predictors for the groups with different etiologies were studied using multinominal logistic regression analysis. Correlations between numeric variables were measured with Paerson’s correlation coefficient. The statistical computation was performed with software (STATISTICA; Statsoft Inc; Tulsa, OK, and a […]

After 2 h of incubation at 37°C, and washing three times with distilled water containing 0.1% polysorbate (Tween) 20 (aqua-Tween), rabbit-antihuman C5 (Behringwerke) diluted 1:200 in 1% BSA-PBS was added for overnight incubation at room temperature. After washing three times with aqua-Tween, antirabbit-IgG antiserum (Orion Diagnostics Espoo, Finland) labeled with alkaline phosphatase diluted 1:300 with […]

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