Archive for the ‘Mesotheliomas’ Category

A high prevalence of virally associated neoplasm has been documented in acquired or congenital immunodeficiency states, specifically Epstein-Barr virus-, human papilloma virus-, and human herpesvirus 8-related tumors. SV40-related lymphomagenesis might, therefore, be more prevalent or easier to detect in such patients. We found a slightly higher percentage of SV40-positive lymphomas in immunocompromised patients than in […]

We found that, overall, SV40 DNA sequences are rarely present in lymphomas from both immunocompetent and immunodeficient patients, and we further confirmed the presence of SV40 in most human mesotheliomas. Thus, SV40 does not appear to be related to the increased incidence of lymphomas observed in the second half of this century, but may be […]

Because of the high homology that exists between the DNA of SV40 and the DNA of the JC or BK viruses, the identity of the band obtained by PCR and visualized by ethidium bromide staining was confirmed by both Southern blot hybridization and DNA sequencing. The results of these analyses confirmed that authentic SV40 was […]

Thermocycling was performed by denaturation for 3 min at 94°C followed by 45 cycles. Each cycle consisted of 1′ at 94°C, 1′ at 60°C, and 1′ at 72°C. For Southern blot analysis, 20 μL of the PCR reaction was loaded onto and run in a 2% agarose gel for 3 h at 100 V. The […]

In this study, we investigated non-Hodgkin’s human lymphomas obtained from nonimmunocompromised patients to determine if they contained SV40 DNA sequences. Because viruses may spread more efficiently and be more pathogenic in immunocompromised individuals, we also tested for SV40 lymphoproliferative disorders that had developed in immunocompromised patients. Materials and Methods DNA Detection Frozen tumor samples were […]

In the last half century, there has been an enormous increase in the incidence of lymphomas and mesotheliomas. A link between SV40 and mesothelioma was established when the tumors were found to develop in hamsters injected intrapleurally, intracardially, and intra-peritoneally with simian virus 40 (SV40). In addition, through polymerase chain reaction (PCR) analysis, RNA in situ hybridization, […]