Archive for the ‘Growth Factor’ Category

The data above suggest a role for IGF-I in development and decidualization of the uterine stroma. Our data suggest that the actions of IGF-I may be modified by IGFBP-4. Croze et al. proposed that in the rat uterus, IGFBP-1 may inhibit the mitogenic activity of IGF-I to allow for the differentiation of stromal tissue. In […]

The expression of IGF-I and IGFBP-4 mRNA in the periimplantation mouse uterus correlates both temporally and anatomically to many of the events of implantation. In the current study, we demonstrate that decidualization of the uterine stroma, in the absence of an embryo, can maintain IGF-I and IGFBP-4 mRNA expression beyond gestational Day 4. As decidualization […]

The results of this study indicate that decidualization of the uterine stroma is necessary for maintaining the expression of IGF-I and IGFBP-4 mRNA in the mouse uterus both during and after implantation of the embryo. Our data reveal that before implantation, the maternal hormones of pregnancy support IGF-I and IGFBP-4 mRNA expression. Beyond gestational Day […]

The extent of decidualization of the oil-injected horn was visualized by staining for alkaline phosphatase activity, an early decidual cell marker. Figure 3 shows a representative deciduomata-induced (A) and control uterine horn (B) stained for alkaline phosphatase activity. In the deciduomata-in-duced horn, a crescent of anti-mesometrial stroma surrounding the luminal epithelium stained intensely for alkaline […]

Previously, we have shown by in situ hybridization that the expression of IGFBP-4, a potential IGF-I inhibitor, correlates to the expression pattern described above for IGF-I mRNA throughout the periimplantation period. To determine whether uterine IGFBP-4 and IGF-I mRNA expression can be demonstrated in the absence of an embryo, in situ hybridization studies were performed […]

In initial experiments, in situ hybridization studies were conducted to localize IGF-I mRNA in the mouse uterus during the periimplantation period. On gestational Day 2, before embryo passage from the oviduct to the uterus, no IGF-I mRNA was detected in mouse uteri (data not shown). IGF-I mRNA was first detected at low levels in uterine […]

The slides were incubated overnight with the appropriate probe in moist chambers at 56°C for IGFBP-4 or 53°C for IGF-I. On the following day, the slides were treated with RNA-se-A and rinsed in a series of washes of increasing stringency. The final wash was in 0.1-strength SSC at 69°C or 66°C for IGFBP-4 or IGF-I, […]