Archive for the ‘Granulosa Cells’ Category

Northern Analysis Total cellular RNA was isolated using the RNeasy Total RNA kit (Quiagen, Chatsworth, CA), and the aliquot of 3 fig was electrophoresed on a 0.66 M formaldehyde-agarose gel. The RNA samples were then transferred onto nylon membrane filters and hybridized at 68°C for 24 h with a 32P-labeled cRNA probe in a solution […]

GC Culture Rats were handled in accordance with the NIH Guide for the Care and Use of Laboratory Animals, and the protocols were approved by the University of Califomia-San Diego (UCSD) Animal Subjects Committee. GC were collected from ovaries of immature DES-primed rats and plated into 12-well dishes (Coming Glass, Coming, NY) that had been […]

Hormones and Reagents Ovine FSH (NIH-FSH-S20, activity 175 X NIH-FSH-Sl) and a monoclonal antibody against IGF-I (anti-IGF-I antibody) were kindly provided by the National Hormone and Pituitary Program (NIDDK). A specific Inh-a antibody, kindly provided by Dr. Nicholas Ling (Neurocrine Biosciences, San Diego, CA), was generated in the rabbit against an amino terminal 30-amino acid […]

Experiments with immature estrogen-primed rats have provided compelling evidence for the involvement of activin receptor signaling in GC differentiation. These GC express type II activin receptor mRNA and possess ~4000 specific activin receptors on the cell surface. Two activin А-dependent functions have been identified in cultured GC: the expression of inhibin-a subunit (Inh-a) and of […]

Activins are members of the transforming growth factor P (TGFp) superfamily of dimeric proteins, which include the TGF(3s, inhibins, activins, Mullerian inhibiting substance, vascular endothelial growth factor, bone morphogenetic protein, and growth and differentiation factors. Activins are composed of two subunits, (ЗА and (3B, that homo- or heterodimerize to generate three different dimeric proteins called […]