The lack of a commercially available vancomycin solution for oral administration poses problems for adults and children who are unable to swallow capsules. Until the time of this study, vancomycin oral liquid was prepared on an “as needed” basis by the pharmacy staff at Fraser Health. These solutions were given short expiration dates, and refrigeration [...]

Regression analysis of the peak area ratios of vancomycin to internal standard versus vancomycin standard concentrations demonstrated linearity over the range of the concentrations, with coefficient of determination (r2) greater than 0.998 (n = 4). The intraday and interday CVs were less than 10% and therefore within acceptable limits: 2.7% and 5.5%, respective­ly, at 0.31 [...]

Preparation of Study Samples The vancomycin study samples were thawed, mixed (by vortex for 10 s), and diluted to 2.5 mg/mL in HPLC-grade water (Fisher Scientific, Richmond, British Columbia; lot 092106). A 0.5-mL aliquot of each sample was then diluted with 0.5 mL of internal standard (metronidazole 1 mg/mL). The final theoretical concentrations were 1.25 [...]

Preparation of Vancomycin Stock solutions of vancomycin 25 mg/mL were prepared by reconstituting commercially available vancomycin for injection (10 g/vial) (Pharmaceutical Partners of Canada, Richmond Hill, Ontario; lot 204369, expiry December 2009) with sterile water for injection (Baxter Corporation, Missis- sauga, Ontario; lot W9F15AO, expiry January 2011) and then diluting with a mixture of equal [...]

INTRODUCTION Orally administered vancomycin is indicated for the treat­ment of Clostridium difficile infections and is often used as the first-line agent in severe cases. With the rising incidence of C. difficile infection in the health care setting, usage of oral vancomycin has also increased. Typically, oral vancomycin is administered in capsule form. However, vancomycin in [...]