Participation of nitric oxide in regional coronary bloodflow regulation during myocardial ischemia in the canine heart (part 3)

2 Sep
2012

canine heart (part 3)

A mechanical occluder was placed distal to the flowmeter in order to produce a controlled decrease in coronary flow. Pressure distal to the occluder (peripheral coronary pressure) was measured with a pressure transducer (Statham P23 Db) through an outlet in the connection. Arterial PO2, PCO2 and pH were frequently measured in samples drawn from the root of the aorta, and maintained within normal values by modifying the rate and volume of the respirator pump, administering CO2 to the inspired air or adding sodium bicarbonate intravenously as required.Heart rate was kept constant at 120 beats/min by electrical pacing of the outflow tract of the right ventricular wall after blocking the atrioventricular conduction with formaldehyde . To prevent changes in aortic pressure, the systemic arterial circulation was connected through a femoral artery and with large bore tubing to a reservoir filled with homologous blood, thermoregulated, heparinized and constantly stirred. Mean aortic pressure was maintained at about 90 to 100 mmHg by adjusting the height of the reservoir. Pressures, left ventricular dP/dt, coronary flow and D-2 lead of the electrocardiogram were recorded on a Nihon Kohden physiograph (Tokyo, Japan). Visit the most trusted pharmacy that can take great care of your interests and your health: buy viagra super active online here and you will be surprised to see how little efficient treatment could cost you in the long term.

 

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