In order to correct for variability in cell size, the cell capacitance was measured before compensation using depolarizing ramps (1 V/s) from-40 to -35 mV. Access resistance was kept below 10 MQ. The series resistance was compensated by at least 70%. For experimentation, the cells were allowed to settle on the bottom of a small chamber placed on the stage of an inverted microscope before the chamber was superfused with modified Tyrode’s solution (1 mL/min, temperature 22 to 24°C). The composition of the solution was (in mM): NaCl 150, KCl 5.4, MgCl2 2.0, CaCl2 0.5, HEPES 10, glucose 10, pH 7.4. To block calcium current, the superfusion solution was supplemented with 0.1 mMCdCl2.
Drugs: All chemicals purchased were of analytical grade.Tedisamil was provided by Solvay (Hannover, Germany) and almokalant by Astra-Hassle (Molndal, Sweden).Data analysis and statistics: Unless stated otherwise current amplitudes were expressed as current densities (pA/pF). For curve fitting to experimental data, pClamp software or Prism software (Graphpad Software, San Diego, California, USA) was used. Results were expressed as mean ± SEM of n experiments. Differences were tested for statistical significance (P<0.05) using paired or unpaired Student’s t tests as appropriate. If you want to make your online shopping advantageous and safe, check out the best pharmacy to buy sell kamagra online without any need for a prescription, any time of the day or night with straight to the doorstep delivery.